FPLC (Fast Protein Liquid Chromatography): Principle, Working, Applications and Advantages
Imagine that you have finally got that E. coli culture growing your favorite enzyme! All you have to do now is lyse them to get a nice turbid, viscous solution of proteins, DNA, lipids, salts, and cellular debris. Your enzyme is just one infinitesimal speck in this giant conglomeration. How will you isolate your protein of interest without denaturing it? This is the problem faced by every biotechnology lab, day in and day out. From an M.Sc. dissertation to a pharma company in Hyderabad to a research institute, protein purification is one task that recurs, with slight variations, in all of them. One of the most common techniques used is FPLC (Fast Protein Liquid Chromatography). In this article, I will be briefly discussing FPLC: the principles, the instrumentation, the working procedure, and a few comparisons. This is the kind of practical detail that I wish I had known when I was first learning about protein purification. 📑 Table of Contents What is FPLC? Principle of FPLC ...
